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Engineering of

Ruizhao Wang,Xiaoli Gu,Mingdong Yao,Caihui Pan,Hong Liu,Wenhai Xiao,Ying Wang,Yingjin Yuan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 89-99 doi: 10.1007/s11705-017-1628-0

Abstract: The conversion of -carotene to astaxanthin is a complex pathway network, in which two steps of hydroxylation and two steps of ketolation are catalyzed by -carotene hydroxylase (CrtZ) and -carotene ketolase (CrtW) respectively. Here, astaxanthin biosynthesis pathway was constructed in by introducing heterologous CrtZ and CrtW into an existing high -carotene producing strain. Both genes and were codon optimized and expressed under the control of constitutive promoters. Through combinatorial expression of CrtZ and CrtW from diverse species, nine strains in dark red were visually chosen from thirty combinations. In all the selected strains, strain SyBE_Sc118060 with CrtW from DC263 and CrtZ from sp. strain PC-1 achieved the highest astaxanthin yield of 3.1 mg/g DCW. Protein phylogenetic analysis shows that the shorter evolutionary distance of CrtW is, the higher astaxanthin titer is. Further, when the promoter of in strain SyBE_Sc118060 was replaced from FBA1p to TEF1p, the astaxanthin yield was increased by 30.4% (from 3.4 to 4.5 mg/g DCW). In the meanwhile, 33.5-fold increase on transcription level and 39.1-fold enhancement on the transcriptional ratio of to were observed at early exponential phase in medium with 4% (w/v) glucose. Otherwise, although the ratio of to were increased at mid-, late-exponential phases in medium with 2% (w/v) glucose, the transcription level of both and were actually decreased during the whole time course, consequently leading to no significant improvement on astaxanthin production. Finally, through high cell density fed-batch fermentation using a carbon source restriction strategy, the production of astaxanthin in a 5-L bioreactor reached to 81.0 mg/L, which was the highest astaxanthin titer reported in yeast. This study provides a reference to greatly enhance desired compounds accumulation by employing the key enzyme(s) in microbes.

Keywords： synthetic biology     astaxanthin     <i>βi>-carotene hydroxylase     <i>βi>-carotene ketolase     <i>Saccharomycescerevisiaei>    

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Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 4,   Pages 806-814 doi: 10.1007/s11705-018-1739-2

Abstract: The deletion of <i>YER151Ci> is proved to be responsible for the increase.

Keywords： synthetic biology     genome rearrangement     prodeoxyviolacein     SCRaMbLE     <i>Saccharomyces cerevisiaei>    

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Exposure-response of Cr(III)-organic complexes to Saccharomyces cerevisiae

Nivedita CHATTERJEE, Zejiao LUO,

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 2,   Pages 196-202 doi: 10.1007/s11783-010-0008-5

Abstract: Hexavalent chromium [Cr(VI)] bioreduction produces soluble Cr(III)-organic complexes. The Cr(III)-organic complexes are relatively stable once they are formed, and no data about their toxicity were reported. Therefore, this study aims to investigate the bioavailability and toxicity of the soluble Cr(III)-organic complexes. L-1 wild type yeast strain was chosen as the model organism and Cr(III)-citrate was selected as the representative compound of the Cr(III)-organic complexes. The short-term chronic aquatic toxicity tests of the Cr(III)-citrate was explored by measuring growth inhibition, direct viable cell count, dry biomass, biosorption, and the amount of CO production. Cr(III)-citrate exerted a toxicity of 51mg/L with an , which was calculated from the percent growth inhibition. These toxicity data would be helpful to define the toxic potential of the organo-chromium-III compounds in the environment.

Keywords： Cr(III)-organic complexes     Saccharomyces cerevisiae     toxicity     EC50     bioavailability    

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Biosorption of Cd

Fengyu ZAN, Shouliang HUO, Beidou XI, Xiulan ZHAO

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 1,   Pages 51-58 doi: 10.1007/s11783-011-0206-9

Abstract: The biosorption of Cd and Cu onto the immobilized ( ) was investigated in this study. Adsorption kinetics, isotherms and the effect of pH were studied. The results indicated that the biosorption of Cd and Cu on the immobilized was fast at initial stage and then became slow. The maximum biosorption of heavy metal ions on immobilized were observed at pH 4 for Cd and Cu . by the pseudo-second-order model described the sorption kinetic data well according to the high correlation coefficient ( ) obtained. The biosorption isotherm was fitted well by the Langmuir model, indicating possible mono-layer biosorption of Cd and Cu on the immobilized . Moreover, the immobilized after the sorption of Cd and Cu could be regenerated and reused.

Keywords： Saccharomyces cerevisiae (S. cerevisiae)     biosorption     heavy metals     immobilization    

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Assembly of biosynthetic pathways in

Lidan Ye,Xiaomei Lv,Hongwei Yu

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 126-132 doi: 10.1007/s11705-016-1597-8

Abstract: A robust and versatile tool for multigene pathway assembly is a key to the biosynthesis of high-value chemicals. Here we report the rapid construction of biosynthetic pathways in using a marker recyclable integrative toolbox (pUMRI) developed in our research group, which has features of ready-to-use, convenient marker recycling, arbitrary element replacement, shuttle plasmid, auxotrophic marker independence, GAL regulation, and decentralized assembly. Functional isoprenoid biosynthesis pathways containing 4–11 genes with lengths ranging from ~10 to ~22 kb were assembled using this toolbox within 1–5 rounds of reiterative recombination. In combination with GAL-regulated metabolic engineering, high production of isoprenoids (e.g., 16.3 mg?g dcw carotenoids) was achieved. These results demonstrate the wide range of application and the efficiency of the pUMRI toolbox in multigene pathway construction of .

Keywords： pathway assembly     toolbox     reiterative recombination     <i>S. cerevisiaei>     biosynthesis    

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Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations Article

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Engineering 2019, Volume 5, Issue 2,   Pages 287-295 doi: 10.1016/j.eng.2018.11.029

Abstract: mdash;from several bacteria, we constructed functional 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) in Saccharomycescerevisiae to take on a role similar to that of the plant-derived cytochrome P450 enzyme and produce

Keywords： Saccharomyces cerevisiae     Caffeic acid     Heterologous enzyme     Cytochrome P450     Synthetic biology    

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Comparative lipidomic analysis of

Bin QIAO, Hong-Chi TIAN, Ying-Jin YUAN

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 4,   Pages 461-469 doi: 10.1007/s11705-012-1223-3

Abstract: Variations in the composition and level of phospholipids (PLs) in yeast cells during industrial ethanol fermentation processes were analyzed. A comparative lipidomic method was used to investigate the changes in total cellular PLs during continuous and fed-batch/batch processes. The phospholipid metabolism in yeast changed during both processes, mainly due to the presence of long-chain poly unsaturated fatty acids (PUFA) that contained phosphatidyglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylserine (PS). The complexity of the media affected the growth of the yeast and the membrane composition. Yeast incorporated lots of exogenous saturated and PUFAs from the feedstock during the fermentations. During the continuous fermentation, there was an increase in PLs with shorter chains as the fermentation progressed and early in process there were more long-chains. During the fed-batch/batch process, the PG species increased as the fermentation progressed. This is probably due to an inositol deficiency in the earlier part of the fermentation.

Keywords： lipidomics     systems biology     phospholipids     stirred-tank     saccharomyces cerevisiae     biorefinery    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Frontiers of Structural and Civil Engineering 2014, Volume 8, Issue 4,   Pages 448-455 doi: 10.1007/s11709-014-0262-x

Abstract: Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

Keywords： <i>JHi> classification     <i>Qi> and <i>RMRi> classification     new method    

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The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era Perspective

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Engineering 2018, Volume 4, Issue 4,   Pages 500-506 doi: 10.1016/j.eng.2018.06.004

Abstract:

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pm) gene Pm21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS·6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. Lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Pm21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat.

Keywords： Wheat     Powdery mildew     <i>Pm21i>     <i>Pm40i>     Alien species     Native resistance    

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NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 11,   Pages 1698-1706 doi: 10.1007/s11705-023-2334-8

Abstract: The electrocatalyst NiFeRuO<i>xi>/NF, comprised of NiFeRuO<i>xi> nanosheetsNiFeRuO<i>xi>/NF displays high electrocatalytic activity and stability for overall alkalineA solar-driven system containing such a bifunctional NiFeRuO<i>xi>/NF has an almost 100%The NiFeRuO<i>xi> coating around Ni foam is an anti-corrosion layer and also a critical

Keywords： NiFeRuO<i>xi> nanosheets     Ni foam     electrocatalysis     overall seawater splitting     solar-driven    

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Presence and Antimicrobial Susceptibility of RE-<i>cmeABCi>-Positive <i>Campylobacteri> Isolated Article

Dejun Liu, Weiwen Liu, Xing Li, Hong Yao, Zhangqi Shen, Yang Wang, Jianzhong Shen

Engineering 2020, Volume 6, Issue 1,   Pages 34-39 doi: 10.1016/j.eng.2019.10.013

Abstract:

Campylobacter spp. (C. spp.) are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of C. spp. that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines, all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positive Campylobacter isolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088 Campylobacter isolates (931 C. coli and 157 C. jejuni) were recovered from the RE-cmeABC screening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%) C. jejuni and 11 (1.2%) C. coli. This multidrug efflux pump is more prevalent among C. jejuni than C. coli. The level of resistance was significantly different in 111 RE-cmeABC-positive C. jejuni versus 46 RE-cmeABC-negative C. jejuni for florfenicol, clindamycin, and erythromycin (P < 0.05), but not for ciprofloxacin (CIP), tetracycline (TET), and gentamicin. However, the isolates harboring RE-cmeABC could shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABC in Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positive C. jejuni isolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABC in Campylobacter of food-animal origin, which would pose a significant threat to public health.

Keywords： RE-<i>cmeABCi>     <i>Campylobacteri>     Ciprofloxacin     Florfenicol    

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A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 3,   Pages 679-686 doi: 10.1007/s11705-020-1979-9

Abstract: A convenient and highly efficient method is described for the synthesis of -methoxycarbazole derivatives, including those with sterically demanding, benzannulated, or strongly electron-donating or -withdrawing substituents. Various -methoxycarbazole derivatives were directly prepared in good-to-moderate yields by the Pd (dba) CHCl /9,9-dimethyl-4,5-bis(diphenylphosphino)xanthene-catalyzed reactions of the corresponding dibromobiphenyl compounds and methoxya-mine. Based on this methodology, the first total synthesis of 3,3′-[oxybis(methylene)]bis(9-methoxy-9 -carbazole), an antimicrobial dimeric carbazole alkaloid previously isolated from the stem bark of , was achieved in 18% yield over seven steps from 1,2-dibromobenzene.

Keywords： <i>Ni>-methoxyl carbazole     dimeric <i>Ni>-methoxyl carbazole     alkaloid     total synthesis     double <i>Ni>-arylation of methoxyamine    

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Thermal and Mechanical Properties Optimization of <i>ABi>O4 Type EuNbO4 By the<i>Bi>-Site Substitution of Ta Article

Lin Chen, Jing Feng

Engineering 2020, Volume 6, Issue 2,   Pages 177-184 doi: 10.1016/j.eng.2019.12.006

Abstract:

Ferroelastic ABO4 type RETaO4 and RENbO4 ceramics (where RE stands for rare earth) are being investigated as promising thermal barrier coatings (TBCs), and the mechanical properties of RETaO4 have been found to be better than those of RENbO4. In this work, B-site substitution of tantalum (Ta) is used to optimize the thermal and mechanical properties of EuNbO4 fabricated through a solid-state reaction (SSR). The crystal structure is clarified by means of X-ray diffraction (XRD) and Raman spectroscopy; and the surface microstructure is surveyed via scanning electronic microscope (SEM). The Young's modulus and the thermal expansion coefficient (TEC) of EuNbO4 are effectively increased; with respective maximum values of 169 GPa and 11.2×10–6 K–1 (at 1200 °C). The thermal conductivity is reduced to 1.52 W·K−1·m−1 (at 700 °C), and the thermal radiation resistance is improved. The relationship between the phonon thermal diffusivity and temperature was established in order to determine the intrinsic phonon thermal conductivity by eliminating the thermal radiation effects. The results indicate that the thermal and mechanical properties of EuNbO4 can be effectually optimized via the B-site substitution of Ta, and that
this proposed material can be applied as a high-temperature structural ceramic in future.

Keywords： Thermal barrier coatings     Rare earth niobates     Substitution     Thermal conductivity     Thermal expansion coefficients     Young’s modulus    

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Traces and echoes of <i>De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

Frontiers of Mechanical Engineering 2016, Volume 11, Issue 1,   Pages 3-11 doi: 10.1007/s11465-015-0359-5

Abstract: the 16th and 17th centuries by analyzing a book of Western technology that he wrote, namely, <i>Taixi Shuifai> (<i>On Western Hydraulicsi>).Taixi Shuifai>.found that Archimedes’ screw and Ctesibius’ machine, which are included in Vitruvius’<i> De Architecturai> volumes, also appear in the work of Xu Guangqi.

Keywords： Vitruvius’ <i>De Architecturai>     Xu Guangqi’s <i>Taixi Shuifai>     Jesuit missionaries     Italian Renaissance    

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Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 5,   Pages 1199-1208 doi: 10.1007/s11709-021-0749-1

Abstract: Shear wave velocity ( <i>Vi> S) is a key parameter in such analysis, although the economyVarious <i>Vi> S–SPT– <i>Ni> correlations are routinely incorporated in seismic hazardanalysis to estimate the value of <i>Vi> S.i> S calculations.The scattering nature of the <i>Vi> S values estimated using different <i>Vi> S

Keywords： uncertainties     <i>Vi> S– <i>Ni> correlations     <i>Vi> S30     SPT data     statistical    

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Title Author Date Type Operation

Engineering of

Ruizhao Wang,Xiaoli Gu,Mingdong Yao,Caihui Pan,Hong Liu,Wenhai Xiao,Ying Wang,Yingjin Yuan

Journal Article

Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan

Journal Article

Exposure-response of Cr(III)-organic complexes to Saccharomyces cerevisiae

Nivedita CHATTERJEE, Zejiao LUO,

Journal Article

Biosorption of Cd

Fengyu ZAN, Shouliang HUO, Beidou XI, Xiulan ZHAO

Journal Article

Assembly of biosynthetic pathways in

Lidan Ye,Xiaomei Lv,Hongwei Yu

Journal Article

Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Journal Article

Comparative lipidomic analysis of

Bin QIAO, Hong-Chi TIAN, Ying-Jin YUAN

Journal Article

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Journal Article

The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Journal Article

NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

Journal Article

Presence and Antimicrobial Susceptibility of RE-<i>cmeABCi>-Positive <i>Campylobacteri> Isolated

Dejun Liu, Weiwen Liu, Xing Li, Hong Yao, Zhangqi Shen, Yang Wang, Jianzhong Shen

Journal Article

A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

Journal Article

Thermal and Mechanical Properties Optimization of <i>ABi>O4 Type EuNbO4 By the<i>Bi>-Site Substitution of Ta

Lin Chen, Jing Feng

Journal Article

Traces and echoes of <i>De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

Journal Article

Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

Journal Article